Faults affect the reliability of the power system network. The frequency and degree of faults determine the downtime (outage time) of a power system network. The more frequent fault occurs, the less reliable the power system is. To ensure a high degree of reliability and to provide quality service at a reduced cost to consumers, protection, and control of fault are required. Successful protection and regulation of faults in the power system demand adequate knowledge and analysis of faults. This research work deals with the analysis of faults in transmission lines using MATLAB/SIMULINK. In this research a 300KM transmission line model was developed, faults simulated and analyzed such as 1L-G, 2L-G, and 3L-G faults during the simulation study of the various faults, the FFT tool was used to analyze the harmonic content of the various faults. The effect of faults was observed and discussed. A discrete powergui with sampling time Ts=5e-05s was used for the simulation study under various fault conditions.